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CRIMIC CR71139

Gene CG15529

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:30152059..30153699	99D9-99E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCACAATCTCCTGGCTC	CATGCCTCTCCTCGATCTCC

Images

Construct Design Sequences

sgRNA sequence	AAGCCAAATCCCCAGAGTGACGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACAGCCTGATGGAAGTCAGATCGCCCACATTCTGTAGCTGCTGCTTCGGTTATCAGCGGCAGGCCAGTCAGGAAGGTGACGTCGCAGAACGGGTTTCCAGCACCGACGACGATCCACAACAGCAGCCCCGTGAGGATTACGTCATTGTGGGTGAGTACGCATCGATCTCTCGGTTTCAAAACAAAGCCAAATCCCCAGAG	TGACGGCATATAATCCAAAACAGAAAGCACAATACAGTTCTTAAAGCTAAATTTAACTCGAATTTAAGTTTAAATGATCGAAATTTGATAAATAATAGATAACTACAGAGGCATAAATTAATGTAGAGTAATACTCAATTAGAAGATTAAGCGGTAAATAGGTCCAATTACTAATACAACATTTTTAATTCCGCAAAGAC

 

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