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CRIMIC CR71140

Gene Cyp12c1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:18684259..18686319	75D6-75D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCATCCACTGTGTTCACGTTC	AGGTGGTTGATGGTGTTCATAAAG

Images

Construct Design Sequences

sgRNA sequence	TTTATCTAAAAGAACGTTATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGAGAAGGTCTATCGCACCGAAGGTCAGTGGCCCGTCCGCGGTGGCGCCGAACCCGTCATCCACTACCGCAATAAGCGGAAGGATGAGTTTTTCAAGAACTGCATGGGATTGTTCGGCAAGTGAGTTTAAATAAATATATTCGCAAAAAATCAACCTACTCATTTAAAGCTTTTTCCATCTATTTATCTAAAAGAACGT	TATTGGCCAATGCAATTCATATATCTCAAATAAAATAATTGAAATTAATAACTCATAACATATGCTGGTTCCTTTAATCGATTTACTTTCAATTTCAGTGGCGCGGAGTGGGGAAAGAATAGAAGTGCCGTGAATCCCGTCCTGATGCAGCACCGAAATGTTGCAATCTATCTGAAACCGATGCAGCGCGTCAACCGGCA

 

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