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CRIMIC CR71141

Gene sad

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:11877235..11879754	87A1-87A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATCCTTAGCCTGAGCCTCTTC	GTAGTTGAACTCTCTTAAGTCGAAGAG

Images

Construct Design Sequences

sgRNA sequence	TCTATAAAATTGGCTTATGCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCTGCCGTCGCCGCCAAATACGTGCCCATTCCAAGGGTGAAGGGACTTCCGGTAGTTGGGACACTTGTGGATCTTATAGCCGCCGGCGGAGCCACGCAGTAAATCCGTAAAGTTGAACCTCGAAAAGTCGGAGAGATAGCAATCGTGTTGAAAATGCATAATATATTCTTGTGTCCGAAAACTCTATAAAATTGGCTTA	TGCCGGACTAGTTCAATGTTAAACAATTCAACCTTAATTGGCAACCTTTTGAAAAAAGCGATTATCAATAGGTTTTTCTTTTAAGAAAGTGCTTAACCCTACTAAATTACCAAAAAGTGCAAAACGTAATAGAGTTTTTTATATCTAAAATGCTTCATGAGATACTAAATTATATTAAAATCTCACAAATCGAAATATTT

 

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