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CRIMIC CR71142

Gene CG34384

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:7814361..7855392	84E1-84E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGGAGACAATCAAGGAGTGAAGTTC	CGAAGACCATGATGCTCCAGC

Images

Construct Design Sequences

sgRNA sequence	TATACACTGCCTCTCCCTCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATTGAATCCTGCACAAGTCTTTGATCTAATCTCTACTGGTCCGAGTCTAGGATTACGGCTCTTCCGACACTTCGAGATGTTTCGAATCCTGGTGTGCTCCGGTGACGGATCCGTAGGCTGGGTTCTTAGCGAGATCGATCGCTTTAACATGCATGTAAGTTTTTATATGTATAAAAATACACTATACACTGCCTCTCCC	TCTTGGAAAAGTATTAGTAAAGACACATTTTACAAAATAGCAAGTCACAGACTGGATTAATTTTACATATGTATTTTATAAAATATTATTTTCTTCTCTAAACGAACAGAAACAATGTCAGGTGGCCGTGATGCCTTTAGGGACGGGCAACGATCTGGCCAGGGTTTTGGGTTGGGGCTCCAGCTGCGACGACGACACCC

 

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