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CRIMIC CR71144

Gene chm

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:7412417..7422736	27F3-27F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAAGCGGAAAATCTGATCCTGC	GAGGTCAAGAAACCGTGGCTG

Images

Construct Design Sequences

sgRNA sequence	TATGCACCGTACATGGGCTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGATTCCTAATTGCTTATTATTTGAATAGTCGCGCTGCAATTGTGCGCCAGAATTTTCCAGAACCTTTATGGTTATTCCCCAGCATAAAATTGAATAATAACATTTTACAGTTTTATGATTCCTTTATGTCTGGAGACGGAATAGAGATGCGAGGAATGTAGAAGCCGGTATCCCAGACTCCCAGAATGGCAGCCACAG	CCCATGTACGGTGCATACATATGTACCGTCAAGCAGACTATATGGTTGTATCCAGATGCAGACAGTGAGCTCTGCCTTGACCATGCCCACCAAAAACAGCAGATGATTTTTCCATTTAATTTTTTTGTCAACGTTTTCGGGTAAATAAGCAACAGACAGAGAGAAACAAGCGGCGCGAACGAGAGAGTCATTTCCCCTGG

 

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