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CRIMIC CR71147

Gene Rpt6

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:21316052..21317663	19F4-19F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGATGGAAATCGAGTCGG	TCTTGTGCAGCGTATAGCTCTCA

Images

Construct Design Sequences

sgRNA sequence	AAACATATAAGACTTGTTCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAAGGCGAGGGATTCCGGTCCTACTACATCCAAAAGATCGAGGAACTGCAGCTGGTGGTGGCCGAGAAGCACCAGAATTTGAGACGACTGCAGGCCCAGCGCAATGAGCTCAATGCTAAAGGTAAATTTACTGTTACACATATGTAGATAATGTGCATAGTTGGTTAGCTGTAGGATATAGAAAACATATAAGACTTGT	TCTTGGTCTTAATATCAAAAAATCTTTAAATTTGAGAAAGCAATCCTAATTAGCACGTCTTTTGCAGTTCGGATGCTGCGGGAGGAGCTGCAGCTGCTCCAGGAACAGGGTAGCTATGTGGGCGAGGTTGTGAAGCCCATGGACAAGAAGAAAGTGCTGGTCAAGGTGCATCCCGAGGGCAAGTTCGTCGTCGATCTGGA

 

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