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CRIMIC CR71148

Gene sordd1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:15793721..15796028	13E18-13F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GGTGCAGCCAATGGGAATGTT	GCTCTTAAGGCAATGGCCATTTC

Images

Construct Design Sequences

sgRNA sequence	CTAAAACTTTACCCGCATCTTGG
sgRNA2 sequence	GCTGGAGTCGTAGGGTATCGCGG

 

left_200bp	right_200bp
CTGCGCTTGAAGTATTTAATTAAATTAAGTCATATTCAAGAAAGTGCGGACCTTAGTTGTACACCAGAAATGGCCTAGGAAAATCATCTAAGCAATCTAGGCACTTGTCAATAGGGTGAACACAGACTTACTTAGCAAATTTGGGTTTCTAAATCATCCAACGTTACGTTGCAGCTTGAACGACTAAAACTTTACCCGCA	TACCCTACGACTCCAGCATAAAAAACTGAAATTTCGAAATCATTGGATCGTAATGATTTAATTCAAATGTAGATGTCAAAAATTGTATAAATATATATTTTAAATTCGATAAAACATTAAAAACATTTATTTCAATTCATTTTCCGTGTAGATTATTATCGATGGGTGATTGGGAGCGATTGAATCAATAGGAATAAAAT

 

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