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CRIMIC CR71151

Gene Ars2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:6080828..6085625	42A9-42A9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	TGCCGATATGTTGTATTCTACGCTCT	CGTTCTGATCTCAGCACGC

Images

Construct Design Sequences

sgRNA sequence	CATCGTCGGAATCACCCATTTGG
sgRNA2 sequence	AAAAGTTGACCGTACAAAACAGG

 

left_200bp	right_200bp
GTAAGCAAACTGACAAGATGGCGGCCACATCTGCTTAATTGAAAATCGAAATTAAATACGATATAACTAGCCTGCCGACCCAAATTGCAAACGGTTTGGAGCTGTTGTAATCATAATATTTGAAGCCCTTACCGGCACATAAGTGAGGTTAAGCACAAGCGACTTTCATCTATATTACTTGCCTTCGTTGCAGACCAAAT	TTGTACGGTCAACTTTTGTATTCCACAAGGAAATAAAGTGCGAAAAAAAGCTTTTTGCTGAGTTTTTATTGGGTTCTATTGTTTTTCCCTTTGTAAACAGAGAGCGCGTAGTATAACGTACGGCAATGTCTAAACGAGATACACTTAAACACATAAGTTCTCTTGGCTAGCCCCGAAAGACTGGCATTTGGAGAAACGAG

 

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