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CRIMIC CR71153

Gene CG2747

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:8016210..8030790	84E8-84E9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTCGAAAATTGGATAAGCTTCCAAG	CTGAATGTTGCTTCCTCCACC

Images

Construct Design Sequences

sgRNA sequence	TTGGTAGTACCTTAGTAACAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATTTTATTTTAAAATACCGATATCGGTTGTTAAGCTTCGATATGCTTTCAGTAAGCGTATTTGTTGTTAATTTTTCAACAATTGCCTTTGATTTTTTGAGAAAATCATTTTAGCAATAAGCCATATCGATTTTGCTTTTTTCTGCATGCATACATTTTGAATAGTTAATTTGAATTGCATTCTTTTTGTCATACCTTGT	TACTAAGGTACTACCAAAGACACTAGAATAATGTCTTTGGTACTACATATTAATTGGTAGCATTACTAATTAAAACTTTATTGTTTTTTTTAGCTGCAACCCAATAGCGCCGCGGGATCTGGCGCCTTAGAGCACGATCCATGCTGTTTGTACCGTCCCAGGTCTGCCCAAGGCAATGGATCCAGCTCGAATGGTTCATC

 

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