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CRIMIC CR71154

Gene CG32683

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:10410391..10454895	9C1-9C4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAGTGGAGATGGATGTCGAG	GCTGGAGATTTCAAAGATTTTACAAGG

Images

Construct Design Sequences

sgRNA sequence	ACAAGGTCGCTCGGAGCGCGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCATCGGGATCAGCAATTGGCGCTGCAGCTGCTGGCGAATCGGATGCACCCGCTCCGGCCAATCCAAATCCGGGGCTGGAACGCGACAGGGACAGGGACTCGCACGGCGACAGGTGAGTGAAGCGAATTCCGATACAGATTCCCATTCCGATAGCCATTCCCGTTCCGAACACTCCATCGACATCCAGCCCATCCTCGC	GCTCCGAGCGACCTTGTCAATATCCATTAGCTAATTGGGCATGCGATGTTGTGGAGGCAAGTCGGGCCAAAAGTTGGGCCTAGTCTGGCCCGAAAAGTGAAGCCAACTGTCCCAAATTAAATCAACACCATTACAATATATCGAATAGCATGCCCATACATACATACATAATTTAATTATATTAACCGATATGTATATAT

 

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