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CRIMIC CR71155

Gene Ance-5

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:24888628..24891033	60E5-60E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTGTCTCTTCTTCGGAAGGAAT	TCATCATCAAGCCATCAGCCCATG

Images

Construct Design Sequences

sgRNA sequence	GCTCGAGCTTTCGTCAGAGTCGG
sgRNA2 sequence	TACAGCACGTACTTATCTACAGG

 

left_200bp	right_200bp
CACTGCGATTTCCGCCTCGGGCTGGCACACTCAGCGTATAAATAATGCCAAGCTCGTAACGTATACGCAACGTTTGCTTGGAGAGATCGCTAGCTGGGTTTCTTTCCCAATTACAGATAAGCTCAATGCCCCGGACTTAGATTGATAAGCCTGCGGCTCGACTGGCAGTCTGGACTCAGTTCTGCTCGAGCTTTCGTCAG	TACAGGTATAAAACTATAAGCCAATTACCTAAGAAGAAATATAAATGAACATAAACAATCAAGTATTAACAATATTTTCTGATATTTAAATAACCGATCCATTATTTTAAGTATTTATTTACAAAGGCACTTTGGTAAAACGACAGAAACTTACGTATAAATTAAGCACTACAGATATCGTTATTTTACTGATCAAGTAT

 

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