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CRIMIC CR71159

Gene Eato

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:13784084..13790562	34D1-34D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGTTCTTCGTCTTTCTGCGG	GCTGCATCTCGAAGCTGTTGTTGT

Images

Construct Design Sequences

sgRNA sequence	ATAAGGATTAGTGTGGTCACAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCAACGCAACTTGCTGCCCCTGCTCCTTATCTGCTCCGGATTTGCCGGAGTACTCACCGCGTACCTCCACTGGTCCGTCAAGCCCGTGCTTCACCAAATTAACCAGTTTGAAGCAAAGAATGAAGTAAGTTTCCCTCGCACTGCTACTTTATCTGCTTTTTATATTTGTACATACGTATGCATGTGATAACAAACCTGTG	ACCACACTAATCCTTATCATTGGACTTCAATTTATTTATTAGATGGTTCTTAGAGAATTGTACTTTCCCGGTAATGAGCTGGATTACATTTACTACTCACCCATCTCTCCCAATGTAGAGGACATTATGGATCTCCTGCGTGTAGATCTGGGAATTATACCAGAGCGTAAGTGCATTTCCAAATTATCTCATAAATTAGA

 

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