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CRIMIC CR71161

Gene TfAP-2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:21586828..21613349	78D7-78E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TAGACTACTGATGCTGATGCGTCG	TGGGTTGGCTACTGGTGATCGTTT

Images

Construct Design Sequences

sgRNA sequence	GAAACACAGAAAGTCTTCTGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGAACAGCTGGATAGCCCCATCGATATGTGTGCATAAGGTTTCGGCGGACTTAAATATGATAACATGACTTTAGCGTATGTGGATATGCCAGCTGTAATTCAGGTTCAGGTATATTTCGGACTTAGGATCTCACTGGGATTAAGATTGATGAGATTGACAGTGTGTCGAATTTCGCGGCGCTTTAAGAACATGGCCTCAG	AAGACTTTCTGTGTTTCGGATCGGATATAGATGGTGCATTCTTGAATGTTATATATATGAGCTCGCAATTTGAGTTCGCTGTGGAACCTCACAATGAAAATATTGAACAAATATTGTGGTTTTGCAATTCAATTAAAAAATGAAATCAATTTTAATAGCAAACCCAATTTTAACCAAAACTGACAATTTTTGGTCGAACT

 

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