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CRIMIC CR71163

Gene bnl

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:19794073..19836817	92B2-92B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAACGGAACCCAGGACGAGAAT	ATACATCCACATCGGCTGAGGAGT

Images

Construct Design Sequences

sgRNA sequence	ATTTGGTCTCGTAAAATGGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTGAATTCAGTAAGTAAAAATGCCAATCTATGAAGAACTTCAATAATTACTTGGATTATGAACTCGTTAGGGGACGAATTCTTTAATTAAGTACAGCTGCTTTTAATAAAACATACGATGATGAAGAACAATATACTTTACACTTCAAAACAGTACTCATATGAACTCTAGATAGCTAACAAAATTTGGTCTCGTAAAAT	GGGAGGTGCTAACCAATAGAATAGTTTTTATCTTCTCCAGATTTTGTTGTTAAAGTTCTATTATTTGATGAACTTCAAAGTGGACGCCAGCACTCCCCATTACTCATCTTGTATTTCGGTTTGAATATGTTTTATGTTCAATTTCTTTGGGGGTTCTTTGTTTGGTTTTTTTGTTTCAAAGTTCGAACCACCCATTCCAC

 

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