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CRIMIC CR71167

Gene Rpp20

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:19744431..19745898	18E5-18E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGTGTTCGCTAGAGTTGGCCAGT	ATTGAGGATCTTGATGTGCAGGGC

Images

Construct Design Sequences

sgRNA sequence	GGACTCTGTCACTTAGCACATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTGATGATGGGAAGCAATTATCCCGAGCACGGGACCAAGCCGAGATCCGCCAAATACCACAAACAACAGAACCACCGAGTGGTGCGCAAGCAGCCGCCGCGTCCCGCTGTTAGCGACCGCCACAACATCTACATCACCAGCAAGACAGACTTTAAGGCAAGTAGAACTGAACTCATGCTGGATGGTGAATCGCCCATGT	GCTAAGTGACAGAGTCCATCTCCTTGACAGGCCCAGCAGCGCCGCTGCGAGGAGCTGATTAACTCCGGCGCCCACGAGATCTTTCTGCATTGCATGGGCTTCTCCGTAACCCGCGGCCTCAATATCGCCCTACGCCTCGTCCAGAATTCTGACGGCGCCCTTAGCTACGCCATCAACACCTCCACCGTTCAGCTGGTGGA

 

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