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CRIMIC CR71168

Gene CG5114

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:14982835..14984486	71A4-71A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTTTACTACCAAGATGAACGTGTC	CGCATGTCACATTCTCCCTAAGAG

Images

Construct Design Sequences

sgRNA sequence	ATGCAGCTTAACTTGCTATTTGG
sgRNA2 sequence	CCGGCGCTGGGCGATTAGTTAGG

 

left_200bp	right_200bp
CCGCTTCAATTTGAAAATTCCGCTCAATTTTTGACATTTGGCAACACTGTATTTTGGCTAAAAAAATAATCGACGAAGTCGATAGTCCCAACAAGCAATCGATGTTTAAAAAACATTGGCGAACGAGTTGCCAGACCATTGATTTTCTAGCACGTGTGATACATTCATTTAATTGTTATTTTTATGCAGCTTAACTTGCT	GTTAGGTTTTAAAGTTGTGTACTGTGCTATATATGTGTAAATAGTAAATACATATTAAAATTTATCTCAGTAGCCTGGTGCAATGAACCCATGTGGCGGTCCTGATACACTTGCCCCATATATGTACAAGAAAAAGGGGGGAGGCACAGGGCTGACTAAAGATAGAACACACAAAGAAGGTTACAGTTTGGGGTAACAAA

 

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