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CRIMIC CR71169

Gene CG9799

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:13385648..13388709	87E8-87E8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCCCGAATCCTCCAATGAAAC	GCATATGGCAGTAGTTTCGTCTTG

Images

Construct Design Sequences

sgRNA sequence	GTTTACGGCCAAAACACGTGTGG
sgRNA2 sequence	TGAACTACAACATAATCTGGTGG

 

left_200bp	right_200bp
ACTTGAAAGCGCATACATAAATATTTCGTAACTTTTAGTTTGCAGTTTTTTGGGATAAATGAAAATCGTTATTTTATTTTTGAAATAATCATTTGGCGCCAAAGTGTAATAAAATACGAATACCCCTTCTAAATTTCATTTAGTATATTTAACCATGGTTAGGTGAATTGTTTTCAAGCTCCGGTCACACTTCGCCACAC	GATTATGTTGTAGTTCATTTTGAAATTTTGTAAATAAATTTACAAAACATTTTACATTTTGAATTTGCTTTTGATGTTATTCCTTGCGACAGGCAGGTCCGCAGATTCATTTATAGGATGCTTTATAATCCTCAACAACTACAAAATCCCTCATTATATCCTTAACGATTTAATGGCATTACCATATATATCCCAAACTA

 

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