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CRIMIC CR71170

Gene Acer

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8521933..8525740	29D4-29D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAGATTGGCAGGCAGGTG	TGACGAAAGTTGGTGGGTGAG

Images

Construct Design Sequences

sgRNA sequence	AGATAAGCGCGGCTTGACCCGGG
sgRNA2 sequence	AGTTTGGGATGGCTCAGTCTAGG

 

left_200bp	right_200bp
TATGAATGCGAAACGGCGTACGCTCTCGCAAACGCTCCTACGAGTATAAAAACCGTTCTCCGTCGCAGCAAGCAGTCAGTATCTCCACAGCAGCAGAGGCGACGGGAAGACAAAGCAATCTGAATTGAATACGTCCTAGTTTTGGGTCCGTATAACAAAAAAATTTATAGTGCAAAGTAGCTGAGATAAGCGCGGCTTGA	CTGAGCCATCCCAAACTTGACTTGAGGTTATTAGAACCCGAAAACAAATATATTGGTTTAGTCTGTCATTAGGAACAAACAAAATAAACTTGATAAGAACGATAATGAATTCACAGCATATGCGCATGGATATTTAAATTGATATTTATTGTATCGATTCATTCGTATCGTTTCGTTTCGTTTTATTAATAAATTGCCAT

 

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