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CRIMIC CR71173

Gene Cdk8

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:9837080..9838670	67C10-67C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTTCAGAATTCAAAAATATTTAGGGGG	CGATACTTGCGTATCGTGACC

Images

Construct Design Sequences

sgRNA sequence	GTTATCGGGAGACAGCTGATTGG
sgRNA2 sequence	TTAGAAGGCTAGAAATTAGTAGG

 

left_200bp	right_200bp
ACAGAAAGTTTTTCTGGCTTTGCTTGTTTGTGGATACAATAGAATTTTTTAAACAGTAAACAAAAAATTAAAAAAAACAAAATTATAAAAAAATGTTTTCTGTTTTAAAAGGCTAGCTATAAAAAAAGCTAGGTATGGCGAGCACTGTTCCCATTTTCCATCCCTGGTTAAGGCTGTGTTATCGTTATCGGGAGACAGCT	AATTTCTAGCCTTCTAAGTATTTAATTTAATGACCTTGATAATAGCAAGTAAAGTGTACGATATAAAACGTTGATTTTTAATGGACAGGGTTTTCTTTATTTAATTTTGTGAACTCAAATTAAGACAAATTCGTTAAAATATCTTTCCGAAGGATATTCAAATATAAATTTGAATGCATGTTCTTTCGTCTCAAAAATGA

 

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