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CRIMIC CR71175

Gene AlaRS

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:8378262..8381764	29B3-29B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGTGGACGGCATTGACATG	GCTTTATTGGGCTTCTGCAAATG

Images

Construct Design Sequences

sgRNA sequence	ACTTCTCAGTGGCATAACTGTGG
sgRNA2 sequence	TCAAAATTGTGAGTAATTGAAGG

 

left_200bp	right_200bp
GCTTTGGCTGATGGCGGCACTCCGGACAACACTGGCCGAGGCTATGTGCTGCGACGCATCCTTCGTCGCGCTGTGCGGTAAGATAACTTAATACTACGTCTTATATACATTATATTACATTAGTCATTAGTCATTATTCTTGATCAATTAAAAAATTACACTCTCTAAGCAATAATAATCTTGACATTATCTTCCCACAG	ATTACTCACAATTTTGATACACAACTATGCTTAATCTTAATCCGTTGATCTGTTTTAAACAGCATTTCAGATGAATTTTTACGGCTATTTATAAAATAAAAGCATTTATACACTAAATTAATTACATAATTGTGTTTGGCTGTATTCGTACATCGTTGAAAAATGATTCGGGCTAGTTCACACTTTAAAAACGGGTCCTT

 

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