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CRIMIC CR71176

Gene CG7275

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:15498959..15500561	71D3-71D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTTGCTATCAGTACTAGGTTGATG	GAAACGTTGCTGCATGGCAAAGCA

Images

Construct Design Sequences

sgRNA sequence	AATAAATGCAATCAGGATGAAGG
sgRNA2 sequence	TGATTGCTAACGACTGTAGTTGG

 

left_200bp	right_200bp
CAGAAGAATTCCCTCCAAAATTTCAAATTTCAGTGTTACCCTATTGGTTTTGCATTAGTGTTACCACACCCACTACTCAACACAATCAGCTGACAGCCGCTCTGTATGGCAACACTGTAGCACGCACATGTTGTTTATGTTCGAAACTCACGATTAATTTTATTTTACGCCGCAGAAAAGCTTAATAAATGCAATCAGGA	AGTTGGATAAGTTTGAATAAAATTGATTTTTGTAAGGAGACTATGTATGTATATATTAAGAAGCCTAATCTCAATTGAGTTTCTCACTTACTATCATTGTTCTCAAGTGACTTTCAGTTTGGTCAAGGCAAACTGAAGTTATCAGTGAATTTGCTTAGTTTAAACTATTGTTTGGCTTTAATTCAGTGATACTAGAAGAA

 

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