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CRIMIC CR71177

Gene Oatp74D

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:17487118..17511693	74D1-74D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCATCCTGAAGCGAAAGCC	CCTTATCACAGGTACAGTTGAGAGC

Images

Construct Design Sequences

sgRNA sequence	GCAGTGGTATTGGTTCATGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACAGCCCTCGTCTACTCGGCGGGCATGATCATCCTGATGTTCATCGGCTGCAGCATGAACGACTTTGCCGGCTACAGGCCAAGCGATGGCAACAGGTGGGTCATCGAATAAGTTCTTGGTTAATTACATTTTAGTTATAATTATATAATAAAAGTAAACTATAAAGATAGCATTTGAAAAGGAAGAGATCCAACCAACA	TGAACCAATACCACTGCCCTCATAAGTATGCAAACTTTTTCCACAACAAGAGTATCCTTTTAATTTTTGGATACATGTACAATTAATATATTGCACTACGTGGGACTGCGGGAAAGTGGGACTATAAGCTAGCTATCAAATCCAAAATGTAATTCGTGTTAAGTCGACTTTTTACACTTGATATACGAAGTAACTCTAGT

 

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