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CRIMIC CR71178

Gene Sh

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:17924307..18063247	16F3-16F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACACCCATCACGTTCATCCACAG	GGTGGAGATGCTTAAGTTCTCAGG

Images

Construct Design Sequences

sgRNA sequence	TTTATCGAATCAGCGTAATGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCCACGTGTTGCCCACTAGAAATTCCACCATTCCCCTACCAATAAAACCAAAACAAATACTAAAACAAAATCCCAACCAAAAATAAACTAACAAAATATGAACAAAAAAACTAAAGCAAATCAAATATAAACAGGACAACAATTTGGTTCGGTTTATTTCTATATTGGTAGCACGGCAAATTTTTTATCGAATCAGCGTA	ATGAGGCATGCAACACGGCAAACAGACCGACACACGCACATTTAACATAGACAATAGAATAGAATTAGACTTGGCTTAAATACAAACATACCAATATATGCATACATACATATGTACACATACAAATTGCGTTGGCTGGAAGTGCGGTTCAAAAAGGGAAAGCCTTTGTACAATGCAATAATAAATTGTCAATAAATCGA

 

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