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CRIMIC CR71180

Gene CCT2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:9089513..9092826	8C8-8C8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTTGTCGCTTTGTTGCGCC	ACCAGATGCGTTTGTGATGC

Images

Construct Design Sequences

sgRNA sequence	GGCGGCTTGCACGTGAGCCATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAAATCGACTAATTACGCAGAGAAAATGGTAAATTTAAAAGTAACAACTATATTATTCGAGTAGTTTACGGTGCGGGCTATTGGCGTTGCGTGAATTCGCCCACTTTTCATTGATAATTGTGGCCAATTCGGCGTTTTCATTTGCCGGGTGTGTGTGTGAACGAAGCGCAAGCCATTTTGACAGGCGGCTTGCACGTGAG	CCATGGACTCCGATTTCGCCTGCCACTTCCAGAACCTTCTCCTGCTGTCCACGGGATCTGGGTGAATTGGAGCTATCACTAGATTGCTGGCCGGGATCGGCTCGGGCAGCTGGCTGAGAACCTTCGAGATGCATTCCACGCCGTTTTTGCTGCCGATGAATCACTCGGCGCCGCTCTCCCTCGTCCGCCACTTTTTGGGG

 

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