CR71181

CRIMIC CR71181

Gene CG5445

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:17282496..17305475	15F7-16A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCATCATGGGCGATGTTATCGAGG	CAGAAGTCCAGATAGCAGCCC

Images

Construct Design Sequences

sgRNA sequence	CGCTATCTTAAATTCCCTCTAGG
sgRNA2 sequence

left_200bp	right_200bp
TCTTATATTTTAACATACACAATTTAGAACAATGCATAATTATTGAATTATGAATAATTTTACATTGTTGTGTTTCATTGCCTCCAGCTTGATGAATACACATTTATAGATTACAAACGTAGATTTTTGAGTTTGTCATGGTGATAAATAACACTTTATAAACTATAAACAGGAAAATTAAAGCGCTATCTTAAATTCCC	TCTAGGGCCTTATCTCAAAAGAACTTAAAGAGACGCTATAAAATTAAAGATATATCTTTGTAAATTAGAGCTAGCTCTCAAGTAGTTCTGATTTCTGTAATATGTTATAAAGTTCAGTTCTCTTTAATATATGATATGAATAGGCCAACTATGTACATCTAGAAACATATTGCTAATAACCCAGTTGAATTTCGTCATAT