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CRIMIC CR71183

Gene CG14194

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:19193555..19196037	18A7-18A7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCGACAACTACATTGGTGGGTAC	ACACCACACAATGGCTCCTACGAT

Images

Construct Design Sequences

sgRNA sequence	CAAATTTGGATGATCCTCTATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTCACCCAGAATTAAAATAGCACGGGGATGCAAGAAAATACAATCATATAACAATACAAGCATATACGACCCATACACAAATATGTGTACGGTGCACCATCGCTTCTTCTAGAGAATAGTCCAGATTTATTGTATACAAGGGTTAGCAAGCAGATCATGAGATTGGATATAGCAATATCGAAGAAATCGTCTGACCATAG	AGGATCATCCAAATTTGGTCACTTAACGAGGATATTAGTTCTCTCGCTATTTTAAATAACTTATCTAAGCTAAATATTTAGATTTTTAATTACTGAACAAGATTTATTGTAGGGCATTAACCTTATAATTGGCCTAGTAGCCTAGTCTAATCGAATGCCTTATCCCAGATTGGCCCTACTGGGCGATTTTCACACCGCTA

 

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