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CRIMIC CR71185

Gene tacc

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:4733654..4749056	82D2-82D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACAACTACAACGATATGGATGAGC	GCCATGTATAGTGTGCCTTAACTATG

Images

Construct Design Sequences

sgRNA sequence	TCACATCAGCGATCAACTAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAATAGTGTGTTTCATTCTAGAACGAGGTCCGAAGACATTGAAAAGAAGCTCAAGGAGGGAGAGCTGCGCGAGGAGGCTCTGATCAAGCGTATTACAGAAAAGGACAAAACAAATGCAAAACTAAAGTAAGCCCTCCCTGATTTTAGTTTTTACTACTAGAGTAGTATTTAGTTTCTGTAACCTGGATCAGTACCACTA	GTTGATCGCTGATGTGACCGGTTTTATTAGCAAGGAAACTCTGTATTATAAATCTTTATGGTATAGCTTGTAAGCTATAACAAGTAGAAAGAAGGAGAGCTTTAATTTTTTACTTAATATAATTTAATTTTAAACTTAAGTTTATTTTATTTGCTAATATAGGAAAGACCTGGTTACTATTTAAACAGGTGTAATAACAA

 

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