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CRIMIC CR71186

Gene l(1)G0196

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:22487180..22508129	20C1-20C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGTCTGCATGAATGTAGGTCTC	GTACTATATTGGTACTATATTGTTGACCCCATG

Images

Construct Design Sequences

sgRNA sequence	ATTTAACAACCGCTAGGAGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAATGCTAATTAATTTGTGGATCGGGTGGCATCTGAACCATATATATTAGGCTTCAGTTGTTCTCCCAAATGTCTGGGATTTTAATTTGAAGGATTCTTAAATGTGTGGCGTTAACCAACACAAACACAAAGTCAGAAAAAGCTTAAAAGCTATAAGTAGTTGATGACTGTCAGATACCTATTAAAGTCACCATCCGCCT	CCTAGCGGTTGTTAAATTTATTGGTTACGACTTTATTACGTCCCGAATTATTATTATTTATCAAAATATACTCTATGAAATTAAACCTATTTCATTAATGTGTAAAATGGTTTTGTCATCTCGGGAGGAATTGAAGAGGAAAACAAGAGAATAGGCTACAGATCTTGACGTTCATACGGACGGACAAACAGACAGACAGG

 

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