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CRIMIC CR71187

Gene CG1662

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:13387563..13389332	11F1-11F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACAAGCAAGGCATTATCGGTCTC	CTTTGACGAACTGGACGTGC

Images

Construct Design Sequences

sgRNA sequence	CGGGCCTTCCATTATGTTAGCGG
sgRNA2 sequence	GCTGGCCAGAGGCTGGCGCTGGG

 

left_200bp	right_200bp
ACGATTGTTAAACTATCGATAACTTAAGCCAACTAGCCCTCGTCGTAACAGCTGTTCCCTACACGTGATTGTTGTTGTTGTTGCTGTTGCAACAACCTTTCAAAAGTCAAATCAGGCAAAAAAATTCGAAAAACAATGTGTATAACTTTCTAAGCTAACTCCAGAACCCCGAAAGAACATCAAATTTTTTTATACCGCTA	GCCAGCCTCTGGCCAGCCTCCTCATTTCCTTGTAACTATTTGTTACCAAATCGCAAACCTTGTAAATACAGACGAGCCCGTGTACAGAGACTAACAAATTGTGAATTATTGTAAATTACCAACCAGTTTGGTAGCACTTGTCGGCTGGCAAACGAGTGGAGAGGCTTAGCCACAAGAGGACGAACTAAACAGAGAGATGG

 

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