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CRIMIC CR71188

Gene spir

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:20311219..20348386	38C5-38C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCTCACGGCTACAGTTAGCGGAAA	TTCCGCATCTGAAGTCAACACC

Images

Construct Design Sequences

sgRNA sequence	TTGGGGTTGGCAGGTTCATTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCCTTGAATTTTTAATTTATTATTTTTTAAATCATGCTGAAAATATTTGAACTAAAATTGATCTATAGTCATAGTTATTAATGTAATAACATTTATTTATTAACGTGCTGCACACAACTGGCAATCTTTAGAATCTATATAGAAGTGGAAATGTTCAATACAACATTGATCAACTAACAATTTGGATTGATAACCTAAT	GAACCTGCCAACCCCAAATGACATTTTATTGTCGTTTTTCCTTACAATTATAGTAACATGTTTTAATCACAAATAATTTATTGTACATTGCACGCAAATGCAATTTGACTACCTTTAAGCTAGCAACCCGTGTATATTCTTTCGACTTTGTGTTAAGTTCTGTATCTTTTGAGCAATATTTCCCATTATTTTTTATGAGT

 

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