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CRIMIC CR71190

Gene VhaM9.7-a

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:4262705..4263464	64A12-64A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGGTTTGCGTCTGAGCTAAGATT	TCAGCATAACTGTGTGGTAGCC

Images

Construct Design Sequences

sgRNA sequence	GCAGTTAGGAAAAAAACGGTAGG
sgRNA2 sequence	CAACCCAATTGTGGCTGGTTAGG

 

left_200bp	right_200bp
TGTAAAAGCAGTTGTTAATTTTAATTTAAAAAAATTGTATTTAAAGTTTAAATTAAAGCTTATTTAATAGCATATAAGTCCGAATCTGTATACACAAAAAAATGGACCTAATTTTGGACATCGACGATAACCACAGGTAGGTAAAATAATCGATGTGCGATAAATATTGATTTTTCGTATACAGCAGTTAGGAAAAAAAC	GTTAGGAAAATAGAGTGACTGCTAACTAAATTATTCTGCAATAATCGTCACATTAGCTGTTTGTTTTACGTTGTAAATAAGAAGTAAAAATATCAAGTGTTCTGTTAACTTCGAATTGTTTAAATATCAGATATTCAATAATAAATTAAATATTTAGATTGCCTTGTAAATAAAATCTATAGGCATCAACTAGTACATCT

 

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