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CRIMIC CR71191

Gene RpS18

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:20263979..20264848	56F11-56F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCATTAGTGATGGACCAGAGTGC	TTTAGGTTGGTGTGCGTCTCGAAC

Images

Construct Design Sequences

sgRNA sequence	TTGCAAATGCCGTTAGCTGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTTCTTTTCTTTTCCCGTTTCCGGTGAGTGGTGTGCTGCCCATTTGATTTTTCCAAAATTCTAAAACAAAATACCTCTTAATTCTTTACAGTGAGGAGTAAATACGAGAATACAAACGGCAAAATGGTGAGTTACCTCAAATATGTGCCAATTTGAACGCCAAACGACAAATTCAATGTGAATTGCAAATGCCGTTAGC	TGTTGGCAAAAATGGCGTGGTTCATATGCATTATACTAATGTTTTTTCTTTGCATTCTTGCAGTCGCTCGTCATCCCAGAGAAGTTCCAGCACATCCTGCGTATCATGAATACGAACATCGACGGCAAGCGCAAGGTTGGCATCGCCATGACCGCCATCAAGGGAGTGGGTCGCCGCTACTCCAACATTGTGCTGAAGAA

 

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