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CRIMIC CR71193

Gene Cyp4ac2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:5266392..5268455	25D2-25D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTTTCACTTTAAGGTGCTGCAGTC	GCTGCTGCATAACTTCTTCAATGG

Images

Construct Design Sequences

sgRNA sequence	ATACATATGTATTTCAAAGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTCAAGTAAGTTTACAAAATAGTTAATGGTTGTTAAGTTGTAAGCACTCTCGATTTTTAGAGAAGAGTGCAATAAACTTGTAAAAGTCCTGCATCAAAGTGTGAATACGGAGTTGGAGCTCAATCAGGTTATTCCTCAGTTTACGCTAAACAATGTTTGCGGTAAGTTCAAGTACATTAATAAAACAATAACGCCAGCT	TTGAAATACATATGTATAACTTGAGAGCAGAATGACCAGCAAATATTGAAATAAATTTGCAATGTTGATTAGTAAATCACAACCATTTTCACTGGCCGTTTATTCAACTATGCAGAGAATTTTAGTGCAATTAATGTAATTGCAGAAACCGCACTGGGAGTAAAACTTGATGACTTGTCGGAGGGTATTCGATATCGCCA

 

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