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CRIMIC CR71194

Gene CG5787

CG5787

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12708792..12712952	33F3-33F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTATTCCTAACTAATTCAATAGCGG	CGGACCTTCCAAATAGGAAGAACG

Images

Construct Design Sequences

sgRNA sequence	AAATCCGGGCAAGGACTATTCGG
sgRNA2 sequence	CTCAACAGCCGAATATGCACTGG

 

left_200bp	right_200bp
CCAACTTCGAATCGGACTTATTACCATTTTGAGCAGCCGGCTTTCATTGCTTTGCAACCCTGGCACCAGACAGTGAAAAATTCGGGGAAAGGAAAGCATTTTTTGTGCCGCGTTTGTTGTGTTGGAAAAAATCGCAGAGAAGCCAGTTAATTGGAAGCAGCGGCGAGTGCTCGACGTAAAGGTAAATCCGGGCAAGGACT	CACTGGACCCATCAAAGGAGGAAATAGAGCGTGGGTTCTTCTTCAGCATTATTTTATGGATTGTATGAGTCTGCATCTAGTTGTATTAGAGACCTTTATTATTGCACACATGAAGAACCATCCTCTTGACTTTAAGAAGTAGATTGTAATTATGTCGCAATGCTTTTACAAATAAATAACTTACCCAGATGGGCGTCTCT

 

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