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CRIMIC CR71195

Gene CG15279

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:14844993..14851749	35B6-35B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CATTGCAGTCAGTTCGAAGATGTC	GCAGAAGGATTCCCATTCCG

Images

Construct Design Sequences

sgRNA sequence	TTGTCATACTCTTGTTGATATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTAAAGACAACGCAACCTTTGAAATTGGTTAAATATACTTTTTGAATTGTAATTTATTTACATTTGTGTATTATAACTGTATTATAATACAGTTTCTTAAAAAAATATTTAATATTGCATGTGTTCCTGACGGACCATTTGATCAAAATCTATTTAGTTCAATCAAAATTCTTTTTTCAATATTAAACTACCCCATAT	CAACAAGAGTATGACAATAGCGAACTGCAGAATCTACATCGTCATTCTGTTCATCAACTCGCCCGCTCGTGTCCCAAATACGCGACCACATCTATAGGTGTCGGAATCGGCCAGGTGATATCTATATCCATGGTGACCACCTACTATGTGGCCATAATGGGCATAACCCTGCGCTATCTGTACGAATCGTTCCGGTCGCC

 

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