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CRIMIC CR71199

Gene cv-d

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:15844466..15853581	89A5-89A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAGTAACCTAGTTGATAATCCCAACTG	CAAGACCAGCTCGCTTTGC

Images

Construct Design Sequences

sgRNA sequence	ATGGATGAACACGAGCGGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAAGAAACCTAGAAGCAACATTGATTAAAGCGTTTTGCGATCATTCAAATATGTTTATATATATTATATATACACATGCACATTTAGTATATATCTATATATATATATATATAAGAAATTCAATTGATAATTGAAGAGTTTATTGCCCGTCCGCGCGAATGCGACATAGATGGCGGGAGGGATGGATGAACACGAGCG	GATGGGAGGAGCATCCATCGAATTGAAGTTCAATTTCCCATTTATCTATTTATCCGGCAGATCTGTTCCTGATCTTCGATGCCGGCCATGCCTTCAGCATCATTGGCCTCAACAAACAGATGCTCTACGAGTACGAGGGCAATGTCCTGGTGGGTGCAAAGCCCCAGGACGAGGGCCACCAGGCGCCACCCACCACCGGA

 

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