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CRIMIC CR71200

Gene CG45078

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:10773339..10777343	86C6-86C6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAATGTAGGGTAACTGGCTTAATTG	CGGTGGGTCAAAGTCATATGTACG

Images

Construct Design Sequences

sgRNA sequence	GCAACGTTGCTTGCACAGTTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATATATATACGTTTGCTGTTTGTGTTCCATCTTAATGCTCCCATTGCACATTACTCACAACCACACTACGTCGTATCAATATGCATATATACATATGTGTATTTAGACTAGGGTGGACCGTTTGGAAAAACAAAATCTAAATTTTCATTACTGCACTTCAATAGAAACCTGTTAAGTAGGCTAGCTTTCTGCAGCCCAAC	TGTGCAAGCAACGTTGCGTATACGTAATATCTGTTTAAGGCTTGTGCAAACCAAAAGAGATGCAGGTCCACCCTAGGGTTCAGACACATGTGTAGCTATGCATCTTGTGTCATAATGTAAGGATGTTCGATGTTATTGGATACAGCTTGACCCACCCAGCACTGCGATCTGATACCAAACTCAACTACTGCCCCAACGAA

 

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