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CRIMIC CR71203

Gene Vajk1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:13920707..13923553	34E4-34E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AATAGTGATCAATAGCCACCATCC	CGATGTCGCTGTGCGCTATAGATT

Images

Construct Design Sequences

sgRNA sequence	TTTACTATTCCACGTTCTTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAAAACGGGATCAGTTCTCGTCCAGTTCTCGAATCGCGTGCCACACGACCTGGGAATACGGATTTCCATTCCCGTTCATCGAAGGATAAACGCAGCAGCATGAAGTCGATGGTGAGTATCGGTGGCAGGACGAATGTGTGCAGTGGAAAAGTGTCTTAAAAGGACTCAGTAATAATCATAGAATGCAGAAATACCTTAA	GAACGTGGAATAGTAAAACATAGTGAAATCAACATTATATGCACAATTTGAAATGCATTTAAACGATACGAATTCTTCTTCAAAACTAACTTTGCAATCCGACATGAACTTTTTGTGATCTAAATATGTTAGAATTTAAAACAATATAGTCAACATGTTTTTCATTAAACCGGAATTCGTTAGTTATATGCAAATTTAGC

 

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