CR71204

CRIMIC CR71204

Gene Boot

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:9092506..9094658	45A2-45A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGTAAATACATACAAGTTATTGCCCCATA	GATTAACTGATTATTACTCTGTTCGTCTCCG

Images

Construct Design Sequences

sgRNA sequence	CACTTGCAGCTCTACAAACGCGG
sgRNA2 sequence	TGCGAATTGCGAGTCGACGCAGG

left_200bp	right_200bp
TTGAGCGTTTTGTAATTATAAGGAGGGGTGTGGCAAAGGTGCTCGACTTGGCTATTGATCCTGATCAAGAATATGCTTTTGTATACTATTTTACACTACGAGTAACGGGTGTAAAAACTCAAAAGCTGACCAGTGCAAAGAAGTTTTACTTCGATTTATCCGGGTGGTAATATTCGATTGCAGCACTTGCAGCTCTACAA	CGCAGGCAAGAAGAAGACGATTTCGACGATGCCCAGTGAATGGCAGGGGTGAATAGTCGCTTTCTGCCTCTTTTTTTTATATATATAACACATATTTCGGCTGATAAGAACAGGCTGGGCCTCTTATCAGTAATGCACAACAAGAAGTACACACGGACGAACGAGATGTGAGAAGCGAAAGCGGACGAAAATAGAAACAA