CR71207

CRIMIC CR71207

Gene 7B2

CG1168

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:5376780..5377975	83A1-83A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCGTGTAACATAAACCTCTAGTCATAC	CACTCTCATCAAACTTGGGTCATC

Images

Construct Design Sequences

sgRNA sequence	GTATATACTAACGTGACGTAGGG
sgRNA2 sequence	CATACACGACGTTTCCATATGGG

left_200bp	right_200bp
AGAGGCCTGAAAACCTCAGCTCCAGCAGTTTGGCGCTAATCTAATTCTCGGCCCCCCACAGGACTGCTTCAACATAAAAGAGCTTTCGAGTACGGCGCATGCGTCCTTTCAGCTGTGGCTGCCATAAGGCAGCTTCGATTGGATCTGCGTATATTTCGCAAATTCAACTGGTAGCCGCCGCACGAAGCACAGAGCCCTAC	TATGGGTGTCTAATACTATTATCTATCATAGCTGGTTATCAATGTTGTCCCGCAAGCGATGTCTAGTAATGTTTCTACCCTTTCCCGAAAATAAAGTTATTCGTATAGTACCAGGGCTCTAAGCCGAAAATATGCGGGTATTGGTCTAAACTTGGGGTAGGGTCTCCACTACCGAAGGTGGGTTCGGATGTAGCTTATCC