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CRIMIC CR71209

Gene alphaSnap

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:20313632..20316577	77B3-77B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGAGAAGAAGTTGACCCAGC	CAGCGAACTGAGGAAGTTTCCAAG

Images

Construct Design Sequences

sgRNA sequence	CGTATACGTATTATGGTGAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAAGGGCTTTCTGGGATCGCTGTTCGGGTGCGTATCAAATCAAAGAAGTTTCGCAATTTCTGTGGGAGTGGGGAAATGGAGCTGTGTCTGGTCTAGCCTAGCATCTCCAGAACCCCACAAGGTACTGAGCCCTATGCCAAATAGCACTTGGATACCAATTCACTATGCTTACTACTTTGTTTTTATGTATATACCCACTC	ACCATAATACGTATACGCAGTTGTGGACTCTACGCCTCCACCCAGAAAGAGAAGAAAATAGCGCAAAAGTGCGACTTACAGAGATATGTTTCAGATATGAAGAACAAAAAGTGTGCAAAATGTCGTTAAAAAATATCCGTAGTACATAATATATGTACACTATGCCATTCGTACAGGTTTCGATGAATCATAGTGCACAA

 

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