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CRIMIC CR71210

Gene Apc

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28832301..28844748	98E5-98E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTATGCACTTCTGTCCGTCCACG	GTTGCTTACAAAGTTTTAGGGTCTACG

Images

Construct Design Sequences

sgRNA sequence	GGCTGCCGCCACCTGAATGTTGG
sgRNA2 sequence	TGGTGGGCGCGCGGAAATAGAGG

 

left_200bp	right_200bp
ATGGAGTGTGTGTAGCAACAACAATGAGAGCCGGGGCAATATAATATATCTGCTGAAAACGCATCTGTATCTTTCTTCATGCACTGGAAATTCCGCAAAGACGACGCCGTGTGCCGCTAACTTTCTAAAAAGATGCAGCGCCAGCTAATCATGATAATGACTTAGCTGCTCATTTGCTATTGTGGCTGCCGCCACCTGAA	TAGAGGGTTAATTTGTTCAGGATAGTATGGATCACAAATTAGCCAAGTTAAAGCGAAAACGGAAGTGGAGATTGTAAGAGGAACAGGACTGTATTTGTCAAGCATTTTTTAGGAACCGAACACGCACAGGTCCACATCCACAAGCCGAGCTAGAGTCAGAGTCAAAATCAAAGAGTTTTTAGGATAGGAGTTAATATACA

 

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