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CRIMIC CR71211

Gene CG11050

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:6611011..6617117	26F1-26F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GAAATCACGGCCAGCACG	GGATGGAAAGGTTGAGTCGG

Images

Construct Design Sequences

sgRNA sequence	TGTAGCAACCAGTTACTTGGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGGTGGCCGCCGCATCGGCCAGTGCCGGCAAGAAGCCCTGCTTCAGCTCGGGCCTCGGCGAGATACTTCAGTTCATGGAGCTCATCGGCAACCTGAAGGTATGTGGAACTGGTTTGCCTGCGTTTTTTCCCGCTCCACTTGCGGGTCATATGCTCGCATCGATTTTTCCCGATGTCCTGCGTGTTTTGATTTTGCCACCA	AGTAACTGGTTGCTACAGAACCTTACTCTTCAAAGAGATAACATTTTGTTATTGTTTATTTGGCTTTTAATGCTAATAACTTATATTAGAGCTATTTAGTGATCAGAAAAATGGAAAAAGTTTATTTATTTAGTATGTTGTTTATTTATGTATAGGTGTTAGCAATTTCACGTGTTATTGTTTATTTGGCTTTTAATGCT

 

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