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CRIMIC CR71212

Gene CG13409

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:22029440..22031900	94A1-94A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACTGGGACTAATGGTCCGCACAA	CTTTCGAGTTGAAATCACCTGGC

Images

Construct Design Sequences

sgRNA sequence	AAAGTCGCACAAACGTCGGACGG
sgRNA2 sequence	TTTGGGTATTCGTTCTGCCGCGG

 

left_200bp	right_200bp
CTCCTCCCTTTGCGAGCTTGGCTTTTATTTACCTTCAGTTGTGATAAGAGTCCGCATGCGCAGTGCATGTGAAATGGCAAACAACTCGACGGATGGTCGGCCCACCACCCAATGGCCCAGCCACCCCCACTGGCAAGCCAAAACATATGCGAGTAGTGCTGGCCAAAATCGATATTTTCCACGAAAGTCGCACAAACGTC	CAGAACGAATACCCAAATGTTCCATAGCGCAATTGAAGTTTTAGTGCTAAATGTGTACAAAGTATTCCCTAATATAATGCATAAATTAACTTAAAATTTCAAAAAATTAATCTCTGGTTGATCAATGAAACTGGAGAGGATCGAAATATGTTTGGGTATTCATGATTAAGCTGAATTCGAAATTTATGTTAAAAAAAAAG

 

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