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CRIMIC CR71213

Gene prd1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11781131..11787014	86E18-86F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACTTCCCGTTGCTGTAACAG	CCTACAAAAGAGCATACCAAAGATCAGC

Images

Construct Design Sequences

sgRNA sequence	TTCCATGTTACTGTCGATCTGGG
sgRNA2 sequence	TCAGCTGCGCTGGGTTAACGTGG

 

left_200bp	right_200bp
CGTTGCCAACCTCTTCTCTTATCTGGCAGCTCTGGCGCGCAATCAGCTGTTCGCGGAACTGGCAACTCTATGAGGTCAAAATATTGATTGTTTCACTTGCCAAGACGTTTTTTTTTGGCAATTCTAAAATGTACAAGTGGCGATAAATCTGCACATAAAACTCTTAAAGCAAGCGTAAAAGGAAACGTAAACCACCCAGA	ACGTGGCTCTGGCCATATATACTCCATTTACAAATAGAAAATTATATTTCCATATACATTACTCAAGCGTTAAATATTCTATCCTGAATACTCAAATAGCTAAAGAACAAAAAGTATTGATATTTATATAGTCTGTATGTATTATGTATTATGATTTTGTAATGCGTGCCTCCGCTTGACTCATTCATTCGTTTGCGCTT

 

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