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CRIMIC CR71214

Gene CG31051

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28519548..28520515	98D3-98D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTTCTGTGCAACGAGTAAAGC	GCGTCTGTCTAACCCTCAATACAG

Images

Construct Design Sequences

sgRNA sequence	GGAGTTCTGGAGTCGTGCAGAGG
sgRNA2 sequence	GCGGCGCAGGTAATGCCAGGCGG

 

left_200bp	right_200bp
AATAAATTAAAACTACATAATAAAGAGTGCATATGTATAAAAGATTAAGCTCAGGGGATGGCCCACAATTTGAAAATGCCACCCCATCTGGCAACGCTTTCGTGGCAAGGGGTTATTGATTTTCCAAAGTTTTCCCAGCAGCAGCTGTTCGTGGAGCCTGCGCCCTAAACTCTGGGGTTGATTGGAGTTCTGGAGTCGTG	AGGCGGAGGAGCGGAGGCATATCATCAGGCGGTTCAGGCACAAATCACGTTATTGACAGGCACTAGCAACCTACGTACTTAATAATAAACTCCATTGCCACTACAGAGCTGTAAACATAGATTTATTTGGCTTAGTAATTCCATTCGATAAATATTAATCAATACTAAAAAGAGAACAGTCTTTTACTTAAACAGGAGAG

 

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