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CRIMIC CR71215

Gene CG4596

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:10873038..10875582	86C7-86C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCTGTTCCCGTTGAAAGTAGAC	CGCTGTGCGAGTGTTAGATCTCTA

Images

Construct Design Sequences

sgRNA sequence	ACACAGCCGCGAAGCGACAAAGG
sgRNA2 sequence	TCACATTCTCACATCCTACATGG

 

left_200bp	right_200bp
TTTAAAATTTTGCTATTGAAATAACAGAATTTTAAAAAGTTAAACATGCCGCACGCGCAGGCGCCAAGTTTTTGTAAACATTGCCGCCCAACCCAAAATGCAACAACAGCAGCCCCCTAAAAACGCCAATGAACGCAAATTCAGCACAAGGACGTGCCAGCCGCCCAGGAAAAATCAATAACAACACAGCCGCGAAGCGA	AGGATGTGAGAATGTGAGGTGTTCCTGGTTCCAGTTTCGTTTCGACCATAAGTGTGATATAAAAAACGTAAATAACATACGCAGATCAACACATTAAGTTTCCTTTGGCCAAATTTGTTCCTTTCAACTTGACATGATCGCGTGTCTCTGCAATTGCAGCTTTAGTGAACGGAAATACAATATATTATATATATAGATAT

 

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