CR71216

CRIMIC CR71216

Gene CG5282

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:20424424..20426071	77C2-77C2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTCGCTTACAAATCTTTTAAGCCC	GTCGTTGAAGGTCTAGTAGACTGTAAGG

Images

Construct Design Sequences

sgRNA sequence	AATCTACGGTAGGAAACCTCGGG
sgRNA2 sequence	AAGAAGTTGGTTCTAAGACTCGG

left_200bp	right_200bp
ATTACATGTTTAAAACACTTTTATCCCGCTCACGCTGGGATGCTGCCATACTAAACAAAATAACTATCCAGCGCGCATGTCCTTGTGAGTAAAATGTGAACACTGAACAGGACACACAGGAAAAATCGATAGTATCCCAGTTTCGAGCTTTCGTCGCAGTGCAAGCTTGTTGAATTGAATCACAATCTACGGTAGGAAAC	CTTAGAACCAACTTCTTTTACAGACTCAAGCGATTGTTCTCACATAATTACTAAAATATTTAATTAAATTATTAAAGTACAAGAGATTTGCTGTACACAAAATTAATAACCGTAAATAGGTAAGTGATGATACCCTGTCTAAGCATAATTTCAAAATTGATTCAAGAGATTACATTTGTACATAGTTTATATTTAATGAA