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CRIMIC CR71216

Gene CG5282

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:20424424..20426071	77C2-77C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTCGCTTACAAATCTTTTAAGCCC	GTCGTTGAAGGTCTAGTAGACTGTAAGG

Images

Construct Design Sequences

sgRNA sequence	AATCTACGGTAGGAAACCTCGGG
sgRNA2 sequence	AAGAAGTTGGTTCTAAGACTCGG

 

left_200bp	right_200bp
ATTACATGTTTAAAACACTTTTATCCCGCTCACGCTGGGATGCTGCCATACTAAACAAAATAACTATCCAGCGCGCATGTCCTTGTGAGTAAAATGTGAACACTGAACAGGACACACAGGAAAAATCGATAGTATCCCAGTTTCGAGCTTTCGTCGCAGTGCAAGCTTGTTGAATTGAATCACAATCTACGGTAGGAAAC	CTTAGAACCAACTTCTTTTACAGACTCAAGCGATTGTTCTCACATAATTACTAAAATATTTAATTAAATTATTAAAGTACAAGAGATTTGCTGTACACAAAATTAATAACCGTAAATAGGTAAGTGATGATACCCTGTCTAAGCATAATTTCAAAATTGATTCAAGAGATTACATTTGTACATAGTTTATATTTAATGAA

 

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