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CRIMIC CR71218

Gene CG8500

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:9784653..9789323	85E9-85E9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTATATCACCATCGGCTTTCGG	TCGTAATTGCAATCGTGGTGCGAC

Images

Construct Design Sequences

sgRNA sequence	CGACCCACTCGAATCCGTATCGG
sgRNA2 sequence	GATGGCATGGCCATGTGACCCGG

 

left_200bp	right_200bp
GGAAAGGTTGTGCGTGCGTGGGCGTTGTGGCCAAGCTGATTTGCTCTTAATTGTTGCAGATAAACGAAAGCATGGCGCCCAAGTAGAACATTCCACCAGCGCCCGACAGCAAGCCAACAAGCTTCAGCTCCTGCTTCTCTCTATTCCGATTTCCACTCCGAGTTTGATATACGCGACCGCGTTCGACCCACTCGAATCCG	ACCCGGGTCACATTGCGTCGAGCTGAAGTGAACGGAGACGACGGTGACAGACAAATACTCGCCTTGGGGCAGCACCCTTGGGTGGGGGGTTGCTGGCTTAGGTAGCCACCCACACGGGCCACGCCACACCACACCACACCACGCACCGCACACACCACTTACACCATACCACATACCACACACAATCCCCACTTTATCCA

 

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