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CRIMIC CR71219

Gene CG9368

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:19601633..19604120	76B9-76B9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCCAGCTTAGTTCGTAGTCCACA	GTTGATTGTGTTGTGCGTGGG

Images

Construct Design Sequences

sgRNA sequence	TCCTTGTAGATACCCATCGCAGG
sgRNA2 sequence	CTTGAAGACCACCAAGTTTATGG

 

left_200bp	right_200bp
AATATTAAGCTACAGTTCTGTAAATAACGAACTGTTGAAATCAACTAACTGCTCTCCCACCCCACAAATCGAAAGCTTGCCCACGCAGTCAGCACGCATGTGCGGGTATGGGCCCCCAAGTGGCTTGCTATTGATTTCTCCAGCGCATTGCTGGGTGAACGCACAAAGAGCTCTGCTCAGCACTCCTTGTAGATACCCAT	TTATGGTGTAAGCCCTGCAAAGGAATCTTAAAATCGCATAGAACAGGGCTGATGGAAAAGCTTTAGCTCTCTACTATATTGTTTGATATACCCAAATGTGATCCCAGATCGTAATGTTAACGAAAACTCAACTCGAAGCACAAGCAACTCTTCTAGGCTATTTATGAAAGGAGAAAACCCCTATGTACGACATTTGTATA

 

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